Fasting and refeeding affect the expression of the Inhibitor of DNA Binding (ID) genes in rainbow trout (Oncorhynchus mykiss) muscle.
نویسندگان
چکیده
The Inhibitor of DNA Binding/Differentiation (ID) proteins are a family of dominant negative regulators of the basic helix-loop-helix (bHLH) transcription factors, shown in mammals to delay cell differentiation and prolong proliferation. In the current study we used real-time PCR to investigate the effects of fasting and refeeding on the expression of ID genes in rainbow trout muscle. Fry shortly following yolk-sac absorption (approximately 250 mg) were used in a pair of experiments. In the first experiment, the treatment groups included fish fed or fasted throughout the duration of the experiment, and fish fasted for 14 days followed by feeding for the remainder of the experiment. The second experiment consisted of the same treatment groups; however the fish were only fasted for 7 days prior to refeeding. In both experiments, ID gene expression in the muscle of fasted fish was significantly lower than the fed samples after 7 days. Refeeding for 3 or 7 days returned the ID expression to levels similar to the fed fish. The reduction of ID expression during a fast and the subsequent return to fed levels with refeeding suggests the ID proteins participate in the regulation of muscle growth in the rainbow trout.
منابع مشابه
Ploidy effects on genes regulating growth mechanisms during fasting and refeeding in juvenile rainbow trout (Oncorhynchus mykiss).
Diploid and triploid rainbow trout weighing approximately 3g were either fed for five weeks, or feed deprived for one week, followed by refeeding. During feed deprivation gastrointestinal somatic index decreased in diploids, but not triploids, and during refeeding, carcass growth rate recovered more quickly in triploids. Although not affected by ploidy, liver ghr2 and igfbp2b expression increas...
متن کاملShort communication:The comparison of fatty acids, fat-soluble vitamins and cholesterol in the muscle of wild caught, cage and pond reared rainbow trout (Oncorhynchus mykiss W., 1792)
متن کامل
Proteomic analysis of muscle tissue from rainbow trout (Oncorhynchus mykiss) fed dietary β-glucan
The aim of this study was to examine the changes in muscle proteome of the rainbow trout fed dietary β-glucan. The experimental diets contained 0 (control), 0.1% and 0.2% β-1,3/1,6 yeast glucan. First, feeding larvae were fed to apparent satiation nine times per day with their respective diets over two months. The percentage of body weight gain and feed efficiency of fish fed 0.2% diet was sign...
متن کاملThe Antioxidant Vitamin (A, C, E) and the Lipid Peroxidation Levels in Some Tissues of Juvenile Rainbow trout (Oncorhynchus mykiss, W. 1792) at Different Oxygen Levels
In this study, the malondialdehyde (MDA) levels in the muscle, liver and kidney tissues and the antioxidant vitamin (A, C, E) levels in the muscle tissue of juvenile rainbow trout were investigated under different oxygen levels (3.5 mg O2/l, 4.5 mg O2/l and 7 mg O2/l) at the end of the 8 hour trial. Fish (initial weight and length, 30.19±2.2 g and 13.15±0.22 cm, respectively) were distributed i...
متن کاملCaspian Sea gamarus (Pontogammarus maeoticus) as a carotenoid source for muscle pigmentation of rainbow trout (Oncorhynchus mykiss)
The red coloration of rainbow trout muscle is an important quality criterion. Duplicate groups of rainbow trout (Oncorhynchus mykiss; initial weight of 200±10g) were fed diets either supplemented 0 (control), 0.5, 2, or 4%, of the Caspian Sea gammarus (Pontogammarus maeoticus) respectively as a prospective alternative carotenoid source. The feeding trial lasted for 39 days. No significant diffe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
دوره 144 4 شماره
صفحات -
تاریخ انتشار 2006